Green synthesis of silver nanoparticles using Lawsonia inermis leaves extract and its Antibacterial activity
Keywords:Silver nanoparticles, Lawsonia inermis, SEM, EDX, Antibacterial activity.
Nanotechnology is defined as a highly developed technology due to the wide range of applications in various fields of medical science, technology and other fields of research. Green synthesis methods are environmentally friendly, readily available, safe, and nontoxic. Nanoparticles are particles with lengths ranging from 1 to 100 nm in two or three dimensions. Silver nanoparticles are important because their unique properties eg, size and shape Depending on the optical, electrical and magnetic properties. In this study, methanolic extract of Lawsonia inermis has been used for synthesis of silver nanoparticles. The change in color from brown to yellowish brown indicates the formation of nanoparticles. To improve the biological composition of natural nanoparticles, many variables were investigated, and these were concentration of the extract, pH and the reaction temperature. The characterization process was performed using techniques of UV-visible spectroscopy, Energy Dispersive X-Ray (EDX) and scanning Electron Microscopy (SEM). The results illustrated that silver nanoparticles had a size of (150-600) nm and a spherical shape, UV-Vis spectroscopy showed SPR sharp peak between (390- 451) nm and EDX attested that Ag, W, Cl and C elements found by weight percent of 57%, 35%, 5.8% and 2.2 % respectively. The antibacterial activity was examined against four types of strain bacteria, two Gram positive bacteria (S. aureus and B. subtilis) and two Gram negative bacteria (E.coli and P. aeruginosa) by using the agar disk diffusion method. The results showed that silver nanoparticles had a strong effect against Gram positive bacteria and it is more than Gram negative bacteria.