Study of Physiological and Histological Changes in Male Rats (Rattus norvegicus) After Carbonated and Energy Beverages Drinking
Keywords:Carbonated beverages, Sperm, Histological changes
This study was conducted to detect the effect of Pepsi cola and Tiger beverages drinking in some physiological parameters and histopathological changes in male rats , thirty two male rats were used which divided into four groups , for each group included eight animals (n=8) the first group administrated physiological saline as control group , the second group administrated (1mL) Pepsi cola , the third group administrated two doses of Pepsi cola by (1mL) in each dose and the duration between these two doses was 4 hours while the fourth group administrated (1mL) Tiger beverage. The results of present study showed a significant increasing (P?0.05) in body weight, sperm malformations, urea concentration, glucose level and triglycerides in treated groups by Pepsi and Tiger beverages compared with control group. Also, the results found a significant decreasing (P?0.05) in the sperm count , ALT , AST, ALP , albumin level and total protein of treated groups (second , third and fourth groups) compared with control group , While observed non-significant increasing in creatinine concentration and cholesterol level of treated groups (second , third and fourth groups) compared with control group .
The results showed that treatment of male rats by Pepsi and Tiger beverages caused histological changes in testes such as necrosis of epithelia , destroyed and increasing the thickness of interstitial tissue , congestion and reduction of spermatocytes . In kidneys that included congestion , infiltration of inflammatory cells , reduce , absence and death of glomeruli , enlargement of Bowman's space and hemorrhage . While in liver that comprised inflammation, congestion, enlargement of sinusoids, hypertrophy of nuclei for hepatocytes and hemorrhage.