The relationship between the loss of GSTM1 and GSTT1 as risk factors for Diabetes mellitus type 2 diabetes of Thi Qar province

Abstract

A total of 96 blood samples were collected from the Center for Diabetes and      Endocrinology in Thi Qar Governorate. The sample consisted of 50 healthy blood samples, including (university teachers, students and others). All samples were aged between 20-65 years. The blood was kept in container tubes on an anticoagulant (EDTA) and kept at a temperature of -20 ° C until it was used to extract DNA from the two groups. GSTM1, GSTT1, which responsible for the Detoxification and Albumin were amplified using PCR polymerization technology.

This study was designed to study the role of Glutathione-S-transferase (Mo-1), theta-1 (glutathione-S-transferase) genes and their contribution to the risk of Type 2 diabetes in Thi Qar Governorate. (39) males and (57) females. A study of some risk factors that increase the percentage of infections that included a number of factors (such as housing, smoking, age, sex, family history, and stress) The prevalence of the disease is higher among urban (55.33%) than in rural areas. The risk of infection among females increases by 59.37% compared to males. patients with a family history had a higher risk compared to those without a family history (77.08%), while the proportion of patients with hypertension was 39.58% compared with the healthy group, while the group of smokers was 28.12% compared to the healthy group. It was found that the highest proportion of patients was among patients aged more than (50) years and was 36.45%) compared to the group of healthy.

The results of the statistical analysis showed that GSTM1 loss increased (OR2.27) compared with the healthy group, while the risk of GSTT1 was increased (OR1.51)  1.51 when compared to the healthy group, while there was no significant difference in the loss of both genes compared to The healthy group.