Association Between BRCA1/2 Polymorphism and HPV in Breast Cancer Patients in Thi-Qar/South Iraq
Keywords:HPV, Breast Cancer, BRCA1/2, Polymorphism, ARMS-PCR.
Breast cancer is presently the greatest common malignant disease detected in women worldwide. The breast cancer occurs as a result of transformations in BRCA1 and BRCA2 when the cells injured, by chemicals in tobacco, exposure to radiation and infected with oncogenic viruses such as a Human papilloma virus, BRCA1 and BRCA2 are human genes which make tumor silencer proteins. This study was carried out to investigate the presence of mutation (5382insC and 6174delT) in BRCA1 and BRCA2 genes, respectively (to determent the effect of HPV of human genes) by gene polymorphism amplification-refractory mutation system-PCR (ARMS-PCR) technique. The ARMS-PCR assay was performed for detection BRCA1 (185delAG) and BRCA2 (6174delT) gene mutation in breast cancer blood patients infected with HPV. The ARMS technique which revealed that out of 32 malignant breast tumors 5 (15.6%) cases carry a 5382insC mutation in BRCA1 gene, and no 6174delT mutation was recorded in BRCA2 gene. The 5382insC polymorphisms recorded in BRCA1 gene, and the HPV may induce it, while no evidence of the BRCA2 6174delT polymorphism, This may indicate that there is no correlation between this mutation and HPV.