The bacteriological study of Acinetobacter baumannii isolated from burn patients in Al- Nasiriyah city - Iraq

Authors

  • N. Abid ٭Dep. Pathological analysis –College of Science - University of Thi-Qar
  • M.kathom Dep. Pathological analysis –College of Science - University of Thi-Qar

Keywords:

Acinetobacter baumannii, Virulence Factors, environmental factors, burn patients

Abstract

The aim of present study was to investigated some virulence factors of Acinetobacter baumannii as well as study their  tolerance to some environmental factors. It was identified 20 isolates of A.baumannii. burn patients in Al-Hussien Teaching Hospital and other burn care units in Al-Nasiriyah city . The specimens were collected during period from August  2018 to February 2019. It were taken by swabs from pus of the burned area and then inoculated on blood agar and MacConkey agar. The isolates were diagnosed by microscopic examination, biochemical tests , API 20 E and Vitek- 2 system . some virulence factors of bacteria detected such as capsule , biofilm formation , colony factor antigens and gelatin liquification , the results revealed that100%, 85% ,75% and 65% of isolates were positive to capsule, biofilm formation , colony factor antigens producing and gelatin liquification respectively .The effect of some environmental factors on bacterial growth are studied .It is found that the optimal range of pH for bacterial growth is 6-10 ,and the optimal growth of bacteria in a concentration 5% of NaCl , as well as , it shows that all bacterial isolates have the ability to grow in 44 °C. Due to the spread of these bacteria in our hospitale ,especially in the burns unite , this study was doen o detecated its properties and virulence factors .

References

 Abdel-El-Haleem, D. (2003). Acinetobacter: environmental and biotechnological applications. African Journal of Biotechnology, 2(4): 71-75.

 Abdulla, A.A.; AL Thahab, A. A.; Abed, T. A.; Mahdi, R. K. and Fadhil, S. (2015). Screening of virulence factors in Acintobacter baumannii isolated from clinical samples. International Journal of Current Research and `Academic Review, 3 (6): 128-134.

 Abston, S.; Blakeney, P. and Desai, M. (2000). Post-burn infection and sepsis. Resident Orientation Manual. Galveston Shriners Burn Hospital and University of Texas Medical Branch Blocker Burn Unit.

 Ahmad, M.; Shahid, H.S.; Ibrahim, K.M. and Malik, S.A.(2006). Pattern of bacterial invasion in burn patients at the Pakistan institutes of medical sciences, Islamabactt. Annals of burns and fire Disasters. 19 (1).

 Braun, G. (2008). Virulence mechanisms of Acinetobacter. In: Bergogne-Berezin E., Friedman H., and Bendinelli M: Acinetobacter biology and pathogenesis. Springer p. 145-155.

 Braun, G. and Vidotto, M. (2004). Evaluation of Adherence, Hemagglutination, and Presence of Genes Codifying for Virulence Factors of Acinetobacter baumannii Causing Urinary Tract Infection.

 Brooks, G. F.; Butel, J. S.; and Morse, S. A. (2004) Jawtz, Melnck and Adelberg, s Medical Microbiology. 23th ed.McGrow-hill. New York .

 Cevahir , N. ; Demir , M . ; Kaleli , I . ; Gurbuz , M . and Tikvesli , S . (2008) Evaluation of biofilm production, gelatinase activity, and mannose-resistant hemagglutination in Acinetobacter baumannii strains . J Microbiol Immunol Infect ., 41:513-518.

 Collee , J.C. ; Fraser , A.G. ; Marmiam , B.P. and Simmon , S.A. (1996). Mackie and McCartney; Practical Medical Microbiology. 24th ed. The Churchill Livingstone. Inc. USA.

 Crouzet, M.; Lesencha, C.; Brozel, V. S.; Costagelioli, P.; Brath, C. and Bonnen, M. (2014). Exploring early steps in biofilmformation: set-up of an experimental system for molecular studies. Bio Med Central Microbiology, 14:253.

 Davey, B. E .and Otoole, G. A. (2000). Microbial Biofilms from ecology to molecular genetics. Microbiology of Molecular Biology Reviews, 64: 847-867.

 Doughari H.J., Ndakidemi P.A., Human, I.S., and Benade S. (2011). The ecology biology and pathogenesis of Acineobacter spp: An overview. Microbes Environ. 26: 101-112.

 Garrity, G. M.; Bell, J. A. J. and Lilburn, T. G. (2005). Taxonomic Outlineof the Prokaryotes, In Bergey’s manual of Systemic Bacteriology. Second Edition. New York Berlin Heidelberg .

 Gohl, O. ; Friedrich, A.; Hoppert, M. & Averhoff, B .(2006) .The thin pill of Acintobacter spp. strains BD413 mediate adhesion to biotic and non biotic surfaces.Appl. Env. Microbiol., 72(2:(1394-1401 .

 Gotz, F. (2002). Staphylococcus and biofilms. Molecular of Microbiology, 43(6): 1367-1378.

 Henwood, C. J.; Gatward, T.; Warner, M.; James, D.; Stockdale, M. W.; Spence, R. P.; Towner, K. J.; Livermore, D. M. and Woodford, N. (2002). Antibiotic resistance among clinical isolates of Acinetobacter in the UK, and invitro evaluation of tigecycline (GAR – 936). Journal of Antimicrobiology Chemotherapy, 49: 479 - 487.

 Hood , M .; Jacobs ,A .C.; Sayood ,K.; Dunman ,P.M.; Skaar ,E.P.; (2010).Acinetobacter baumannii increases tolerance to antibiotics in response to monovalent cations. Mar;54(3):1029-41. doi: 10.1128/AAC.00963-09. Epub 2009 Dec 22.

 Hrenovic ,J.; Durn ,G., Goic-Barisic ,I.; Kovacic, A .(2014).Occurrence of an Environmental Acinetobacter baumannii Strain Similar to a Clinical Isolate in Paleosol from Croatia.;DOI: 10.1128/AEM.00312-14.

 Hrenovic,J . and Ivankovic, I. (2009). Survival of Escherichia coli and Acinetobacter junii at various concentrations of sodium chloride. DOI: 10.5053/ejobios.2009.3.0.18

 Ishii , S.I.; Koki , J.; Unno,H.; and Hori , K.(2004) Tow morphological types of cell appendage on a strongly adhesive bacterium , Acinetobacter sp . strain Tol5 . Appl. Env. Microbiol., 7(8): 5026 – 5029 .

 Jasim ,E. I.(2014). Comparative study on the effect of chloroxylenol and sodium chloride on Brucella and Acinetobacter growth.J.A.B.R, VOL. 4(3): 280-282 ISSN 2250 – 3579

 Kanemitsu, K.; Nishino, T.; Kunishima, H. and Okmura, N. (2001). Quantitative determination of gelatinase activity among Enterococci. Microbiology Methods, 47: 11- 6.

 Karah, N., (2011). Identification, molecular epidemiology, and antibiotic resistance characterization of Acinetobacter spp. clinical isolates. 109.

 King ,L. B., and Swiatlo ,E., et al. (2009). Serum resistance and biofilm formation in clinical isolates of Acinetobacter baumannii. FEMS Immunol Med Microbiol; 55: 414-421.

 klausen, M.; Heydorn, A.; Ragas, P.; Lambertsen, L.; Aaes-Jorgensen, A.; Molin, S. and Nielsen, T. (2003). Biofilm formation by Pseudomonas aeruginosa wild type, flagella and type IV pili mutant. Molecular Microbiology, 48(6): 1511-1524.

 Lewis, K. (2010). Persister cells. Annual review of microbiology, 64:357-372.

 MacFaddin, J. F. (2000). Biochemical tests for identification of medical bacteria (3rd ed.), Lippincott Williams and Wilkins, USA.

 Nahar, A.; Anwar, S.; Abu-Saleh, A. and Amin, M. (2012). Virulence factors and Antibiotic Susceptibility Pattern of Acinetobacter Species In a tertiary care Hospital in Bangladesh. Ibrahim Medical College Journal, 6(1): 27-30.

 Peymani ,A.; Nahaei ,M. R.; Farajnia ,S., et al. (2011).High prevalence of metallo-b-lactamase-producing Acinetobacter baumannii in a teaching hospital in Tabriz, Iran. Japanese Journal of Infectious Diseases.64:69–71.

 Poirel, L.; Naas, T. and Nordmann, P. (2010). Diversity, epidemiology and genetics of Class D100 ß-lactamases. Antimicrob. Agents Chemother. 54:24-38.

 Ramos, G.; Rocha, J. and Tuon, F. (2013). Seasonal humidity may influence Pseudomonas aeruginosa hospital acquired infection rates .International Journal of Infectious Disease, 17: 757-761.

 Rao, R. S.; Karthika, R. U.; Singh, S. P. ;Shashikala, P.; Kanungo, R.; Jayachandran, S. and Prashanth, K. (2008). Correlation between biofilm production and multiple drug resistance in imipenem resistant clinical isolates of Acinetobacter baumannii.Indian Journal of Medical Microbiology, 26: 333-337.

 Sharma, G.; Rao, S.; Bansal, A.; Dang, S.; Gupta, S. and Gabrani, R. (2014). Pseudomonas aeruginosa biofilm: potential therapeutic targets.Journal of Biologicals,42(1):1-7.

 Tomaras ,A.P.; Dorsey ,C.W.; Edelmann ,R.E. and Actis ,L.A. (2003). Attachment to and biofilm formation on abiotic surfaces by Acinetobacter baumannii: involvement of a novel chaperone-usher pili assembly system. Microbiology, 149: 3473-3484.

 Valli, B. and Gopinath, P. (2016). Detection of Gelatinase and Lipase among clinical isolates of Acinetobacter baumannii. Journal of chemical and pharmaceutical sciences, 9(4):3245-324.

 Zhang, Y. L.; Lau, Y .L.; Arakawa, E. and Leung, K. Y. (2003).Detection and genetic analysis of group II capsule in Aeromonas hydrophila. Microbiology.149 :1051 – 1060.

Downloads

Published

2020-08-28

Issue

Section

Articles