Effect of Different Doses of Pumpkin Seed Oil on Liver Function in Adult Male Rats Treated with Chlorpyrifos

Abstract

They present study aimed to investigate the effect of effect of different doses of pumpkin seed oil on liver function in  adult male rats treated with chlorpyrifos . Fifty adult male rats were used and randomly divided into five equal groups of 10 rats each group.Group 1(control) were given corn oil (1ml /kg.bw) , whereas animals of Group 2 were given  CPF 6.7 mg/kg bw in corn oil. Group 3, 4 and 5 were given CPF (6.7mg /kg.bw) plus PSO (20, 40, and 80) mg/kg.bw respectively. The treatment were given once daily by oral gavages for 8 weeks. At the end of the experiment, animals were sacrificed and serum isolated from blood samples were used for measurement of liver enzymes and liver samples were taken for histopathological study. Chlorpyrifos treated group recorded significant increase(p<0.05)in aspartate aminotransferase(AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) and histopathological examination of liver revealed marked congestion in the centrilobular area with hepatocellular necrosis .On other hand groups treated with 20,40and 80 mg pumpkin seed oil  induced improved of these parameters