Determination influence of Vitamin D3 on Beta cell of Diabetic Rats.

Abstract

     Vitamin D has long been recognized for its beneficial role in regulating  the secretion of insulin from pancreatic beta cells (Holick, 2011). The  study included animal experimental during the period extended from November until December to investigate the role of the administration of vitamin D3 on beta cell of pancreas in alloxan induced toxic in mature male rats. 48 male rats were divided into 4 groups contain 12 animals in each as the following ; Group(1) Animals of this group administer D.W  (0.2ml/kg)/day by gavages for 6 weeks orally as control group , Group(2) were injected intraperitoneally with 150mg/kg b.w of alloxan as diabetic group, Group (3)  were injected with alloxan then administer 500 UI/kg/day of alpha 25-hydroxy vitamin D3 by gavages for 6 weeks orally, Group (4)were injected with alloxan then administer 1000 UI/kg/day of alpha 25-hydroxy vitamin D3 by gavages for 6 weeks orally. Cytological and histopathological examination of alloxan treated males showed that marked destruction in  beta cells of pancreas and  sever vacoulation of the cells in the Islets of Langerhans while a significant improvement in Cytological and histological changes of pancreas of alloxan- treated groups with two doses of  Vit.D3 compared with control sections.Our study suggests that 1, 25-(OH)2 D3 could play protective roles in pancreas. through reducing the toxicity  of  alloxan .